![]() Capto adhere ImpRes in bind-and-elute mode. HiTrap Capto Q ImpRes is a strong anion exchanger prepacked with BioProcess Capto ImpRes media for screening and small-scale protein purifications using ion exchange chromatography (IEX).īecause of poor resolution between the monomer and aggregates, MAb3 could not be purified in flow-through mode on Capto adhere ImpRes, and hence, bind-and-elute mode was evaluated. InertSustainSwift C18 Preparative Columns, Particle Size 5µm, I.D. Capto adhere ImpRes is a strong anion exchange multimodal BioProcess chromatography medium (resin), designed to meet the demands of modern large-scale manufacturers, for high-resolution polishing of monoclonal antibodies (MAbs) and other biomolecules.Inertsil ODS-4 Capillary Columns, Particle Size 5µm, I.D.High-throughput medium for intermediate and polishing protein purification, excellent choice for process-scale purification. InertSustainSwift C18 Capillary Columns, Particle Size 3µm, I.D. The differences in chromatographic behaviour of individual deoxynucleotides as well as small single-stranded and double-stranded DNA molecules have been examined for two resins from the Capto family: Capto Adhere and Capto Q ImpRes. Ultra C8 Columns (USP L7), Particle Size 5 μm, Length 250 mm, ID 4.6 mm, Restek (CAT#: STEM-C-0822-LC) High-resolution intermediate purification and polishing based on the well-established Capto platform with traditional ligands Shop Cytiva HiScreen Capto Cytiva HiScreen Capto Q ImpRes and Capto SP ImpRes Capto SP ImpRes Ionic Capac.: 0.16-0.Capto MMC ImpRes is a chromatographic medium based on a multimodal cation exchange ligand (Fig 1). Inertsil ODS-SP Cartridge Guard Column E, Replacement Cartridge E (2/pk) & Holder Set, Particle Size 3µm, I.D.The ligand constitutes a hydrophobic part, a weak cation exchange group, and groups that can promote hydrogen bonds. Rtx-1 Columns (Fused Silica), Length 15 m, ID 0.32 mm, df 0.25 μm, Temp.Ĭapto MMC ImpRes comprises small ( 40 m) beads relative to the related multimodal cation exchange medium, Capto MMC ( 75 m).Capto Q ImpRes: Strong anion (quaternary amine group) exchange BioProcess medium Capto SP ImpRes: Strong cation (sulfonate group) exchange BioProcess medium. Inertsil ODS-HL Guard Columns For UHPLC, Replacement Cartridge (2/pk) & Holder Set For UHPLC, Particle Size 3µm, I.D.HiTrap column prepacked with Capto Q ImpRes resin. InertSustainSwift C18 Capillary Columns, Particle Size 5µm, I.D.Designed to offer quality and flexibility in process-scale applications. Scaling up allows for high resolution combined with quick flow rates. ![]() The similar selectivity of the two columns ensures a smooth transition even for quality control (QC) applications.Capto Q ImpRes resin couples a quarternary ammonium (Q) strong anion exchanger to a chemically modified, high-flow agarose matrix. ![]() Similar resin selectivity and slightly improved resolution can be expected with the Capto HiRes columns while using the same experimental conditions. Capto Q HiRes and Capto S HiRes replace MonoBeads columnsĪ separation that worked on a Mono Q or Mono S column may be performed on a Capto HiRes Q or Capto HiRes S column with little modification or optimization. Protein analysisĬapto HiRes ion exchange columns can be used for protein analysis, in applications such as characterization of target protein charge variants. Capto HiRes columns separate proteins and biomolecules that are very close in net charge, which makes these columns great for these applications. High-resolution separation of samples based on their charge properties is essential to secure sample charge homogeneity and success of the study. In many research areas, for example in structural biology using X-ray crystallography or cryo-electron microscopy (cryo-EM), obtaining homogeneous size and charge of biomolecules is crucial for the elucidation of their structures. The columns have fittings for simple connection to ÄKTA chromatography systems or equivalent high-performance systems. A high-performance IEX column formatĬapto HiRes Q and Capto HiRes S IEX resins are prepacked in high-performance Tricorn glass columns of two different sizes. ![]() Capto HiRes Q and Capto HiRes S are strong anion and strong cation exchangers, respectively with ion exchange properties that remain constant during chromatography within the recommended pH range of 2 to 12. ![]()
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